Plasmid
leghemoglo

Part:BBa_K4232009:Design

Designed by: Songyuan Kong   Group: iGEM22_BIT-China   (2022-10-03)


This is a plasmid used to transport oxygen within a cell


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5135
    Illegal suffix found in sequence at 443
    Illegal PstI site found at 116
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5135
    Illegal NheI site found at 2867
    Illegal SpeI site found at 444
    Illegal PstI site found at 116
    Illegal PstI site found at 458
    Illegal NotI site found at 451
    Illegal NotI site found at 5141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5135
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5135
    Illegal suffix found in sequence at 444
    Illegal PstI site found at 116
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5135
    Illegal XbaI site found at 5150
    Illegal SpeI site found at 444
    Illegal PstI site found at 116
    Illegal PstI site found at 458
    Illegal NgoMIV site found at 2761
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NONE


Source

Legumoglobin comes from soy rhizobia and FRT is obtained from procurement

References